Genetic engineering of Trichoderma reesei cellulases and their production

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Genetic engineering of Trichoderma reesei cellulases and their production

Lignocellulosic biomass, which mainly consists of cellulose, hemicellulose and lignin, is the most abundant renewable source for production of biofuel and biorefinery products. The industrial use of plant biomass involves mechanical milling or chipping, followed by chemical or physicochemical pretreatment steps to make the material more susceptible to enzymatic hydrolysis. Thereby the cost of e...

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Production of Trichoderma reesei cellulases on glucose-containing media.

The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The ...

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Trichoderma reesei strains for production of cellulases for the textile industry

Trichoderma reesei is a biotechnically important filamentous fungus used commercially in enzyme production. T. reesei is also one of the best known cellulolytic organisms, producing readily and in large quantities a complete set of extracellular cellulases for the degradation of crystalline cellulose. In addition to T. reesei, a wide variety of other bacteria and fungi also produce cellulolytic...

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The mesophilic filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) has become a major cell factory in the enzyme industry, and the benchmark organism for production of cellulases, especially for biomass conversion. The strain was originally isolated during the second World War from US Army tent canvas in the Solomon Islands and initially identified as Trichoderma viride. The is...

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ژورنال

عنوان ژورنال: Microbial Biotechnology

سال: 2017

ISSN: 1751-7915,1751-7915

DOI: 10.1111/1751-7915.12726